Soad Yaish-Bachari; Hossein Zolgharnein; Mehdi Mohammadi; Mohammad Ali Salari-AliAbadi; Seied Ahmad Ghasemi
Volume 11, Issue 3 , November 2012, , Pages 62-70
Abstract
We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by ...
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We collected 69 samples of Mudskipper from dominant spices periophthalmus waltoni for genetic study from Hendijan, Khore Zangi and Delvar. A specimen of fin was fixed in Ethanol alcohol (96%) and moved to Biotechnology lab in center study of Persian Golf in Bushehr. Total genomic DNA was extracted by proteinase-K digestion, phenol: chloroform: isoamyl alcohol extraction and ethanol precipitation. The quality and quantity of DNA was assessed using 1% agarose gel electrophoresis and spectrophotomeric method. Polymerase chain reactions (PCR) were conducted on the target DNA using 6 primers RAPD. PCR products were separated by electrophoresis on polyacrylamid gels (8%) that were stained using silver nitrate. Allele frequency, the effective and real allele, expected and observed heterozygosis, genetic diversity, Shannon Information index, Gst and gene based on analysis of molecular variance (AMOVA) were calculated using the GENALEX and Pop Gene software. The average of Shannon index for Hendijan, Khore zangi and Delvar was respectively 0.3887, 0.2860 and 0.3509. Highest of Gst observed between Hendijan and Khore zangi (0.260) and lowest between Khore zangi and Delvar (0.195). Highest of gene flow (Nm) was observed between Delvar and Khore zangi (1.033) and lowest between Hendijan and Khore zangi (0.687). Highest of Genetic identity observed between Delvar and Khore zangi (0.924) but lowest was between Hendijan and Khore zangi community (0.833). Eventually this study show that three community of our research are separate and belong to two clusters.
Shaghayegh Rouholahi; Hossein Zolgharnein; Sohrab Rezavani Gilkolaei; Mohammad Ali Salari Aliabadi; Samira Mohamadian; Mohammad Javad Taghavi; Faramarz laluei; Hojat Shojaei
Volume 10, Issue 2 , September 2012, , Pages 50-58
Abstract
The Mendelian Inheritance of produced hybrids from Chalcalburnus chalcoides and Vimba vimba persa was investigated using Microsatellite Markers. Genomic DNA from the produced larva and also small piece of dorsal and pectoral fin of their parents were extracted by Phenol-Chlorophorm method and PCR reaction ...
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The Mendelian Inheritance of produced hybrids from Chalcalburnus chalcoides and Vimba vimba persa was investigated using Microsatellite Markers. Genomic DNA from the produced larva and also small piece of dorsal and pectoral fin of their parents were extracted by Phenol-Chlorophorm method and PCR reaction was accomplished. 10 loci were amplified, in which 7 of them were amplified with reasonable polymorphism and 3 were monomorphism.
In order to study the segregation of the produced F1 hybrids from female Chalcalburnus chalcoides and male Vimba vimba persa, seven loci (LCO3,LCO1,Lid11,Rru2,Z1-2,Z7-8,Z9-10) were used.
According to the results it showed that parents and F1 larva‘s allelic distribution were almost segregated based on Mendelian inheritance in 7 loci. The present study showed that Microsatellite markers are useful marker in study of inheritance.